4.1 Pre-lab: Intro to RNA-seq
4.1.1 Purpose
In this pre-lab, students learn about RNA-sequencing so that we can take a closer look at some RNA-seq data in class.
4.1.2 Learning Objectives
- Compare and contrast the genome and the transcriptome
- Describe the steps involved in RNA-seq
- Define bioinformatics and its role in biology
4.1.3 Introduction
Next-generation DNA sequencing has revolutionized biological research. This tutorial will explain the basic process of next-gen sequencing and will discuss some of the ways it is used in research.
4.1.4 Activity 1 - Biotechnology: Next-Gen Sequencing
Estimated time: 20 min
4.1.4.1 Instructions
Access the C-MOOR Tutorials
- If you are using SciServer, log into SciServer, click on compute and open your “C-MOOR LearnR” container. Visit SciServer Guides and FAQs if you need to jog your memory on how to do this.
- If you are using AnVIL, log into AnVIL, navigate to your class Workspace, start up an RStudio Cloud Environment, and open RStudio. Visit the AnVIL Guides and FAQs if you need to jog your memory on how to do this. This module can be found in the “6-biotech-ngs” folder of the “rnaseq” curriculum folder.
- If you are using an alternative setup, follow the instructions provided by your instructor.
Start the “Biotechnology: Next-Gen Sequencing” tutorial
To move through the activities click “Continue” at the bottom of the screen. When you are done with a topic, click “Next Topic” to move on.
4.1.5 Activity 2 - Biotechnology: RNA-Seq
Estimated time: 10 min
4.1.5.1 Instructions
- Start the “Biotechnology: RNA-Seq” tutorial. On AnVIL, this module can be found in the “7-biotech-rnaseq” folder of the “rnaseq” curriculum folder.
- To move through the activities click “Continue” at the bottom of the screen. When you are done with a topic, click “Next Topic” to move on.